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PUBLICATIONS

2023

Polyoxazoline-Based Nanovaccine Synergizes with Tumor-Associated Macrophage Targeting and Anti-PD-1 Immunotherapy against Solid Tumors

Ana I. Matos, Carina Peres, Barbara Carreira, Liane I. F. Moura, Rita C. Acúrcio, Theresa Vogel, Erik Wegener, Filipa Ribeiro, Marta B. Afonso, Fábio M. F. Santos, Águeda Martínez-Barriocanal, Diego Arango, Ana S. Viana, Pedro M. P. Góis, Liana C. Silva, Cecília M. P. Rodrigues, Luis Graca, Rainer Jordan, Ronit Satchi-Fainaro, Helena F. Florindo (2023), “Polyoxazoline-Based Nanovaccine Synergizes with Tumor-Associated Macrophage Targeting and Anti-PD-1 Immunotherapy against Solid Tumors”, Adv Sci, 230029 doi.org/10.1002/advs.202300299

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Cell lipid droplet heterogeneity and altered biophysical properties induced by cell stress and metabolic imbalance

Ana E. Ventura, Sarka Pokorna, Natalie Huhn, T.C.B.Santos, M.Prieto, A.H.Futerman, L.C.Silva*, (2023) “Cell lipid droplet heterogeneity and altered biophysical properties induced by cell stress and metabolic imbalance”, Biochim Biophys Acta, Mol Cell Biol Lipids 1868 (9):159347, doi.org/10.1016/j.bbalip.2023.159347

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Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers

Martinho N, Marquês JMT, Todoriko I, Prieto M, de Almeida RFM, Silva LC* (2023). Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers. Mol Pharm. 20(2):918-928. doi: 10.1021/acs.molpharmaceut.2c00321

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RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease

Marta B. Afonso, Tawhidul Islam, Julie Magusto, Ricardo Amorim, Véronique Lenoir, Rui Simões, Liana C. Silva, Rui E. Castro, Paulo J. Oliveira, Carina Prip-Buus, Vlad Ratziu, Jérémie Gautheron, Cecília M. P. Rodrigues (2023) “RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease”, Hepatology, 77(4):1319-1334 doi.org/10.1002/hep.32756

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2022

Editorial “Bioactive Sphingolipids in health and disease”

M Aslan, Y Oztas and Liana C. Silva (2022), Editorial “Bioactive Sphingolipids in health and disease”, Frontiers in Molecular Biosciences, 9, 1051449 10.3389/fmolb.2022.1051449

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Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements

Sarka Pokorna, Ana E. Ventura, Tânia C.B. Santos, Martin Hof, Manuel Prieto, Anthony H. Futerman, Liana C. Silva* (2022), Journal of Photochemistry and Photobiology B: Biology
Volume 228, 112404, doi.org/10.1016/j.jphotobiol.2022.112404

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2021

The long chain base unsaturation has a stronger impact on 1-deoxy(methyl)-sphingolipids biophysical properties than the structure of its C1 functional group

Tania C B Santos, Essa M Saied, Christoph Arenz, Aleksander Fedorov , Manuel Prieto, Liana C Silva* (2021), Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1863, Issue 8, 183628, doi.org/10.1016/j.bbamem.2021.183628

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Biophysical impact of sphingosine and other abnormal lipid accumulation in Niemann-Pick disease type C cell models

Ana C. Carreira, Sarka Pokorna, Ana E. Ventura, Mathew W.Walke, Anthony H. Futerman, Emyr Lloyd-Evans, Rodrigo F.M. de Almeida, Liana C. Silva* (2021), Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1866(8), 158944, doi.org/10.1016/j.bbalip.2021.158944

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NBD derived diphenyl(aminomethyl)phosphane – a new fluorescent dye for imaging of low pH regions and lipid membranes in living cells

Rodrigo F.M. de Almeida, Tânia C.B. Santos, Liana C. Silva, Jakub Suchodolski, Anna Krasowska, Kamila Stokowa-Sołtys, Małgorzata Puchalska, Radosław Starostaa (2021), Dyes and Pigments, in press, doi: 10.1016/j.dyepig.2020.108771

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Biophysical analysis of lipid domains by fluorescence microscopy

Ana E. Ventura, Tânia C. B. Santos, Joaquim T. Marquês, Rodrigo F.M. de Almeida, Liana C. Silva*(2021), In: Bieberich E. (eds) Lipid Rafts. Methods in Molecular Biology, vol 2187. Humana, New York, NY. doi: 10.1007/978-1-0716-0814-2_13

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Biophysical analysis of lipid domains in mammalian and yeast membranes by fluorescence spectroscopy

Carla Sousa, Filipa C. Santos, Andreia Bento-Oliveira, Beatriz Mestre, Liana C. Silva, Rodrigo F. M. de Almeida (2021), In: Bieberich E. (eds) Lipid Rafts. Methods in Molecular Biology, vol 2187. Humana, New York, NY. doi: 10.1007/978-1-0716-0814-2_14

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2020

Lipid domain formation and membrane shaping by C24-ceramide

Ana E. Ventura, A.R.P. Varela, T. Dingjan, T. C. B. Santos, A. Fedorov, A.H. Futerman, M. Prieto, Liana C. Silva* (2020), Biochim Biophys Acta, 1862 (10):183400, doi: 10.1016/j.bbamem.2020.183400

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Canonical and 1-Deoxy(methyl) Sphingoid Bases: Tackling the Effect of the Lipid Structure on Membrane Biophysical Properties

Tânia C.B. Santos, Alexandra Vaz, Ana E. Ventura, Essa M. Saied, Christoph Arenz, Aleksander Fedorov, Manuel Prieto, Liana C. Silva (2020), Langmuir 2020, 36, 21, 6007–6016, doi: 10.1021/acs.langmuir.0c01000

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The role of ceramide in regulating endoplasmic reticulum function

ID Zelnik, AE Ventura, J Kim, LC Silva, AH Futerman (2020) , Mol Cell Biol Lipids, 1865, S1388-1981(19)30118-0 doi: 10.1016/j.bbalip.2019.06.015

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2019

Meeting Report – The 2019 FEBS special meeting on sphingolipid biology: sphingolipids in physiology and pathology

Giovanni D'Angelo, Christopher J. Clarke, Liana C. Silva (2019) , J Cell Sci, 132: jcs235705 doi: 10.1242/jcs.235705

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Ceramide domains in health and disease: a biophysical perspective

AE Ventura, B Mestre and Liana C. Silva*, (2019), In: J Stiban (Eds) “Bioactive ceramides in health and disease: Intertwined roles of enigmatic lipids”, book series: Advances in Experimental Medicine and Biology, vol 1159, Springer Nature, Switzerland, ISBN 978-3-030-21162-2

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Mammalian sphingoid bases: biophysical, physiological and pathological properties

AC Carreira, TC Santos, MA Lone, E Zupancic, E Lloyd-Evans, RFM de Almeida, T Hornemann, LC Silva (2019), Prog Lipid Research, 75:100988, doi: 10.1016/j.plipres.2019.100988

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1-deoxy-sphingolipids

M. A. Lone, T. Santos, I. Alecu, L.C. Silva, T. Hornemann (2019) , Biochim Biophys Acta – Mol Cell Biol Lipids, 1864(4):512-521, doi:10.1016/j.bbalip.2018.12.013

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Cisplatin-Membrane Interactions and Their Influence on Platinum Complexes Activity and Toxicity

N Martinho, TC Santos, HF Florindo, LC Silva (2019), Frontiers in Physiology, 9, doi: 10.3389/fphys.2018.01898

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2018

N,O‐Iminoboronates: Reversible Iminoboronates with Improved Stability for Cancer Cells Targeted Delivery

R. Lopes, A. Ventura, L. Silva, H. Faustino, P.M.P. Góis (2018), Chem Eur J, 24(48):12495-12499 doi:10.1002/chem.20180215

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Functional Moieties for Intracellular Traffic of Nanomaterials

Ana Luísa Silva, Liane Moura, Bárbara Carreira, João Conniot, Ana I Matos, Carina Peres, Vanessa Sainz, Liana C. Silva, Rogério S. Gaspar, Helena F. Florindo (2018), In: Bruno Sarmento, José das Neves (Eds) “Biomedical Applications of Functionalized Nanomaterials”, Chapter 14, Elsevier, Philadelphia, United States, ISBN: 9780323508780; doi:10.1016/B978-0-323-50878-0.00014-8

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2017

The molecular mechanism of Nystatin action is dependent on the membrane biophysical properties and lipid composition

A. G. dos Santos, J. T. Marquês, A. C. Carreira, I. R. Castro, A. S. Viana, M.-P. Mingeot-Leclercq, R. F. M. de Almeida and L. C. Silva (2017), Phys Chem Chem Phys, 19, 30078-30088, doi:10.1039/C7CP05353

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Rational design of novel, fluorescent, tagged glutamic acid dendrimers with different terminal groups and in silico analysis of their properties

Martinho N, Silva LC, Florindo HF, Brocchini S, Zloh M, Barata TS (2017), Int J Nanomedicine, 12, 7053–7073, doi:10.2147/IJN.S135475

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Practical computational toolkits for dendrimers and dendrons structure design

Martinho N, Silva LC, Florindo HF, Brocchini S, Barata T, Zloh M (2017), J Comput Aided Mol Des, 9, 817-827, doi: 10.1007/s10822-017-0041-6

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Poly-glutamic dendrimer-based conjugates for cancer vaccination – a computational design for targeted delivery of antigens

Moura LIF, Martinho N, Silva LC, Barata TS, Brocchini S, Florindo HF, Zloh M. (2017), J Drug Target, 10, 1-8. doi: 10.1080/1061186X.2017.1363213

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Changes in membrane biophysical properties induced by the Budesonide/Hydroxypropyl-β-cyclodextrin complex

Dos Santos AG, Bayiha JC, Dufour G, Cataldo D, Evrard B, Silva LC, Deleu M, Mingeot-Leclercq MP (2017), Biochim Biophys Acta, 1859(10),1930-1940. doi: 10.1016/j.bbamem.2017.06.010

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Development of lysosome-mimicking vesicles to study the effect of abnormal accumulation of sphingosine on membrane properties

Ana C Carreira, Rodrigo F.M. de Almeida, Liana C. Silva (2017), Sci Rep. 7(1), 3949 doi: 10.1038/s41598-017-04125-6.

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Pathological levels of glucosylceramide change the biophysical properties of artificial and cell membranes

Ana R.P. Varela, Ana E. Ventura, Ana C. Carreira, Alexander Fedorov, Anthony H. Futerman, Manuel Prieto, Liana C. Silva (2017), Phys Chem Chem Phys, 19, 340-346 doi: 10.1039/C6CP07227E

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2016

Glucosylceramide reorganizes cholesterol-containing domains in a fluid phospholipid membrane

Ana R.P. Varela, André Sá Couto, Alexander Fedorov, Anthony H. Futerman, Manuel Prieto, Liana C. Silva (2016), Biophys J, 110 (3): 612-622. doi: 10.1016/j.bpj.2015.12.019

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A Three-Component Assembly Pomoted by Boronic Acids Delivers a Modular Fluorophore Platform (BASHY Dyes)

Fábio M.F. Santos, João N. Rosa, Nuno R. Candeias, Cátia P. Carvalho, Ana I. Matos, Ana E. Ventura, Helena F. Florindo, Liana C. Silva, Uwe Pischel, Pedro M.P. Góis (2016), Chem Eur J, 22 (5): 1631-1637, doi: 10.1002/chem.201503943

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Drugs, delivery systems and membrane organization in model and cell membranes

Ana E. Ventura, Tânia Santos, Ana. C. Carreira, Nuno M. Martinho, J. Conniot, Ana R. Varela, Rogério Gaspar, Helena F. Florindo, Liana C. Silva (2016), In: Angel Catala (Eds) “Membrane organization and lipid rafts in the cell and artificial membranes”, Chapter 4, Nova Science Publishers Inc, New York, ISBN: 978-1-63484-589-2

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Regulatory development of nanotechnology-based vaccines

H.F. Florindo, J. Lopes, Liana C. Silva, M.L. Corvo, M.B. Martins, R. Gaspar (2016), In: Micro and Nanotechnology in Vaccine Development, edited by Dr Mariusz Skwarczynski and Professor Istvan Toth, Elsevier ISBN: 978-0-323-39981-4

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2015

Regulatory aspects on nanomedicine

Vanessa Sainz, João Conniot, Ana I Matos, Carina Peres, Eva Zupancic, Liane Moura, Liana C Silva, Helena F Florindo, Rogério S Gaspar (2015), Biochem Biophys Res Commun, 468 (3): 504-510. doi:10.1016/j.bbrc.2015.08.023

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Tackling the biophysical properties of sphingolipids to decipher their biological roles

Ana C. Carreira, Ana E. Ventura, Ana R.P. Varela, Liana C. Silva, (2015), Biol Chem, 396 (6-7): 597-609, doi: 10.1515/hsz-2014-0283

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2014

Cancer immunotherapy: role of nanodelivery approaches for combined targeting and tracking of immune cells

João Conniot, Joana M. Silva, Joana G. Fernandes, Liana C. Silva, Rogério Gaspar, Steve Brocchini, Helena Florindo, Teresa Barata, (2014), Frontiers in Chemistry, Research Topic: Cancer Nanotheranostics: What have we learned so far? Front. Chem, 2:105. doi: 10.3389/fchem.2014.00105

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Molecular Modeling for Dendrimers' Design and Biomedical Applications

Nuno Martinho, Helena Florindo, Liana Silva, Steve Brocchini, Mire Zloh and Teresa Barata (2014), Molecules, 19 (12), 20424-20467 doi: 10.3390/molecules191220424.

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Development of Functionalized Nanoparticles for Vaccine Delivery to Dendritic Cells: a Mechanistic Approach

Joana M. Silva, Gaëlle Vandermeulen, Vanessa G. Oliveira, Sandra N. Pinto, Catarina Rodrigues, Carlos A. Afonso, Christine Jérôme, Liana C. Silva, Luis Graça, Véronique Préat, Helena F. Florindo (2014), Nanomedicines, 9 (17), 2639-2669 doi: 10.2217/mnm.14.135

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Biophysical Implications of sphingosine accumulation in membrane properties at neutral and acidic pH

Eva Zupancic, Ana Carreira, Rodrigo F. M. de Almeida, Liana C. Silva (2014), J. Phys. Chem. B, 118 (18): 4858-4866 doi: 10.1021/jp501167f

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Influence of intracellular membrane pH on sphingolipid organization and membrane biophysical properties

Ana R.P. Varela, Amélia M.P.S. Gonçalves da Silva, Alexander Fedorov, Anthony H. Futerman, Manuel Prieto, Liana C. Silva, (2014), Langmuir , 30: 4094-4104 doi: 10.1021/la5003397

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Ceramide: a simple sphingolipid with unique biophysical properties

Bruno M. Castro, Manuel Prieto, Liana C. Silva (2014), Prog Lipid Res, 54: 53-67 doi: 10.1016/j.plipres.2014.01.004

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Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain

Sandra N. Pinto, Elad L. Laviad, Johnny Stiban, Samuel L. Kelly, Alfred H. Merrill, Manuel Prieto, Anthony H. Futerman, Liana C. Silva, (2014), J. Lip. Res., 55: 53-61 doi: 10.1194/jlr.M042002.

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Translational aspects of nano-oncologicals

R. Gaspar, H.F. Florindo, Liana C. Silva, M. Videira, L. Corvo, B. Martins, B. Silva-Lima (2014), In: M.J. Alonso & M. Garcia-Fuentes (Eds) “Nano-oncologicals: new targeting and delivery approaches”, Chapter 15, Controlled Release Society and Springer, US, ISBN: 978-3-319-08083-3

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Biophysics of lipid rafts and their interplay with ceramide. Studies in models systems and biological insights

Rodrigo F.M. de Almeida, Joaquim T. Marquês and Liana C. Silva (2014), In: Daniel Sillence (Eds) “Lipid Rafts: Properties, Role in Signal transduction”, Chapter 2, Nova Science Publishers Inc, New York, ISBN: 978-1-62948-964-3

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2013

A combined fluorescence spectroscopy, confocal and 2-photon microscopy to re-evaluate the properties of sphingolipid domains

Sandra N. Pinto, Fábio Fernandes, Alexander Fedorov, Anthony H. Futerman, Liana C. Silva*, Manuel Prieto* (2013), Biochim. Biophys. Acta, 1828 (9): 2099-2110 doi: 10.1016/j.bbamem.2013.05.011

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Effect of glucosylceramide on the biophysical properties of fluid membranes

Ana R.P. Varela, Amélia M.P.S. Gonçalves da Silva, Alexander Fedorov, Anthony H. Futerman, Manuel Prieto & Liana C. Silva (2013), Biochim. Biophys. Acta, 1828 (3): 1122-1130 doi: 10.1016/j.bbamem.2012.11.018

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Immunomodulation against microbial pathogens through dendritic cells

J. Silva, E. Zupancic, C. Peres, L.C. Silva, R. Gaspar, V. Préat and H F Florindo (2013), in “Microbial pathogens and strategies for combating them: science, technology and education”, A. Méndez-Vilas(Eds), Formatex Research Center, ISBN (13) Volume 3: 978-84-942134-1-0

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2012

Ablation of Ceramide Synthase 2 strongly affects biophysical properties of membranes

Liana C. Silva, Oshrit Ben-David, Yael Pewzner-Jung, Elad L. Laviad, Johnny Stiban, Sibali Bandyopadhyay, Alfred H. Merrill Jr., Manuel Prieto & Anthony H. Futerman (2012), J. Lip. Res., 53: 430-436 doi:10.1194/jlr.M022715

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Methylation of glycosylated sphingolipid modulates membrane lipid topography and pathogenicity of Cryptococcus neoformans

Arpita Singh, Haitao Wang, Liana C. Silva, Chongzheng Na, Manuel Prieto, Anthony H. Futerman, Chiara Luberto & Maurizio Del Poeta, (2012), Cell. Microbiol., 54, 500-516 doi: 10.1111/j.1462-5822.2011.01735.x.

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2011

Effect of ceramide structure on membrane biophysical properties: the role of acyl chain length and unsaturation

Sandra N Pinto, Liana C Silva, Anthony H Futerman & Manuel Prieto (2011), Biochim Biophys Acta, 1808 (11): 2753-60. doi: 10.1016/j.bbamem.2011.07.023.

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2010

A critical role for Ceramide Synthase 2 in liver homeostasis: I. Alterations in lipid metabolic pathways

YP-Jung, H Park, EL Laviad, LC Silva, S Lahiri, J Stiban, RE-Roman, B Brüger, T Sachsenheimer, F Wieland, M Prieto, AH Merrill Jr & AH Futerman (2010), J. Biol. Chem., 285: 10902-10. doi: 10.1074/jbc.M109.077594

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2009

Cholesterol-rich fluid membranes solubilize ceramide domains. Implications for the structure and dynamics of mammalian intracellular and plasma membranes

BM Castro, LC Silva, A Fedorov, RFM de Almeida & M. Prieto (2009), J. Biol. Chem 284: 22978-87. doi: 10.1074/jbc.M109.026567.

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Lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations

Liana C. Silva, Anthony H. Futerman and Manuel Prieto (2009), Biophy. J., 96 (8): 3210-22. doi:10.1016/j.bpj.2008.12.3923

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FRET analysis of domain formation and properties in complex membrane systems

Luís Loura, Rodrigo F.M. de Almeida, Liana C. Silva and Manuel Prieto (2009), Biochim. Biophys. Acta., 1788 (1), 209-224. doi:10.1016/j.bbamem.2008.10.012.

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2008

Ceramide-containing membranes: the interface between biophysics and biology

Johnny Stiban, Liana C. Silva and Anthony H Futerman (2008), Trends Glycosci. Glycotechnol., 20, 297-313. doi: 10.4052/tigg.20.297.

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Membrane domain formation, interdigitation and morphological alterations induced by the very long chain asymmetric C24:1 ceramide

Sandra N. Pinto, Liana C. Silva, Rodrigo F.M. de Almeida and Manuel Prieto (2008), Biophys J., 95, 2867-2879. doi:10.1529/biophysj.108.129858.

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2007

Formation of ceramide/sphingomyelin gel domains in the presence of an unsaturated phospholipids: a quantitative multiprobe approach

Bruno M. Castro, Rodrigo F.M. de Almeida, Liana C. Silva, Alexander Fedorov and Manuel Prieto (2007), Biophys. J., 93, 1639-1650. doi:10.1529/biophysj.107.107714.

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Ceramide-domain formation and collapse in lipid rafts: membrane reorganization by an apoptotic lipid

Liana C. Silva, Rodrigo F.M. de Almeida, Bruno M. Castro, Alexander Fedorov and Manuel Prieto (2007), Biophys. J., 92 (2), 502-516. doi:10.1529/biophysj.106.091876.

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2006

Nystatin-induced lipid vesicles permeabilization is strongly dependent on sterol structure

Liana Silva, Ana Coutinho, Alexander Fedorov and Manuel Prieto (2006), Biochim. Biophys. Acta, 1758 (4), 452-459. doi: 10.1016/j.bbamem.2006.03.008.

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Competitive binding of cholesterol and ergosterol to the polyene antibiotic nystatin. A fluorescence study

Liana Silva, Ana Coutinho, Alexander Fedorov and Manuel Prieto (2006), Biophys. J., 90 (10), 3625-3631. doi:10.1529/biophysj.105.075408.

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Ceramide-platform formation and -induced biophysical changes in a fluid phospholipid membrane

Liana Silva, Rodrigo F.M. de Almeida, Alexander Fedorov, António P.A. Matos and Manuel Prieto (2006), Mol. Memb. Biol., 23 (2), 137-148. doi:10.1080/09687860500439474.

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2004

Cholesterol and ergosterol influence nystatin surface aggregation: Relation to pore formation

Ana Coutinho, Liana Silva, Alexander Fedorov and Manuel Prieto (2004), Biophys. J., 87: 3264-3276. doi:10.1529/biophysj.104.044883.

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2003

Conformation and self-assembly of a nystatin nitrobenzoxadiazole derivative in lipid membranes

Liana Silva, Ana Coutinho, Alexander Fedorov and Manuel Prieto (2003), Biochim. Biophys. Acta, 1617 (1-2): 69-79. doi: 10.1016/j.bbamem.2003.09.004

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Solution conformation of a nitrobenzoxadiazole derivative of the polyene antibiotic Nystatin. A FRET study

Liana Silva, Ana Coutinho, Alexander Fedorov and Manuel Prieto (2003), J. Photochem. Photobiol. B, 72 (1-3): 17-26. doi: 10.1016/j.jphotobiol.2003.08.005

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